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Anticarcinogenic Effect of Probiotic Dahi and Piroxicam on DMH-induced Colorectal Carcinogenesis in Wistar Rats

Dheeraj Mohania, Vinod Kumar Kansal, Renu Sagwal, Dilip Shah

Abstract


This study examined the therapeutic effects of probiotic Dahi containing Lactobacillus acidophilus LaVK2 and Lactobacillus plantarum Lp9, alone or as well as in combination with piroxicam (PXC) as an antioxidant agent in male Wistar rats administered 1, 2-dimethylhydrazine dihydrochloride (DMH). Colorectal carcinogenesis was induced by injecting DMH subcutaneously (40 mg/kg body weight) twice a week for 2 weeks. The rats were fed with buffalo milk or probiotic Dahi alone or in combination with PXC in addition to basal diet ad libitum and euthanized at 8th, 16th and 32nd week of the experiment and examined for biochemical and histopathological profile. A significant reduction in lipid peroxidation measured as thiobarbituric acid reactive substances (TBARS) in liver and colorectal tissue, and carcinogen activating enzyme measured as b-glucuronidase activity in faeces were observed in probiotic Dahi-DMH-PXC group compared with the DMH control group. Probiotic Dahi alone or in combination with PXC was showed a significant (P < 0.05) protective effect by lowering the levels of TBARS, faecal b-glucuronidase and by enhancing the activity GST in liver and colorectal tissues, correlated with decreased tumor incidence, tumor multiplicity and tumor volume in gastrointestinal (GI) tract in DMH induced rats. These observations suggest that probiotic Dahi alone or conjointly with PXC may have therapeutic potential in attenuation of carcinogenesis in GI tract.


Keywords


Probiotics; Piroxicam; Lactobacillus; Colorectal carcinogenesis

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References


Center MM, Jemal A, Smith RA, Ward E. Worldwide variations in colorectal cancer. CA Cancer J Clin. 2009, 59:366-378

Janne PA, Mayer RJ. Chemoprevention of colorectal cancer. N Engl J Med. 2000, 342:1960-1968

Roller M, Pietro Femia A, Caderni G, Rechkemmer G, Watzl B. Intestinal immunity of rats with colon cancer is modulated by oligofructose-enriched inulin combined with lactobacillus rhamnosus and bifidobacterium lactis. Br J Nutr. 2004, 92:931-938

Horie H, Zeisig M, Hirayama K, Midtvedt T, Moller L, Rafter J. Probiotic mixture decreases DNA adduct formation in colonic epithelium induced by the food mutagen 2-amino-9h-pyrido[2,3-b]indole in a human-flora associated mouse model. Eur J Cancer Prev. 2003, 12:101-107

Corpet DE, Tache S. Most effective colon cancer chemopreventive agents in rats: A systematic review of aberrant crypt foci and tumor data, ranked by potency. Nutr Cancer. 2002, 43:1-21

Pollard M, Luckert PH. Effect of piroxicam on primary intestinal tumors induced in rats by n-methylnitrosourea. Cancer Lett. 1984, 25:117-121

Pollard M, Luckert PH, Schmidt MA. The suppressive effect of piroxicam on autochthonous intestinal tumors in the rat. Cancer Lett. 1983, 21:57-61

Reddy BS, Nayini J, Tokumo K, Rigotty J, Zang E, Kelloff G. Chemoprevention of colon carcinogenesis by concurrent administration of piroxicam, a nonsteroidal antiinflammatory drug with d,l-alpha-difluoromethylornithine, an ornithine decarboxylase inhibitor, in diet. Cancer Res. 1990, 50:2562-2568

Pereira MA, Barnes LH, Steele VE, Kelloff GV, Lubet RA. Piroxicam-induced regression of azoxymethane-induced aberrant crypt foci and prevention of colon cancer in rats. Carcinogenesis. 1996, 17:373-376

van Lieshout EM, Tiemessen DM, Peters WH, Jansen JB. Effects of nonsteroidal anti-inflammatory drugs on glutathione s-transferases of the rat digestive tract. Carcinogenesis. 1997, 18:485-490

Kumar M, Nagpal R, Kumar R, Hemalatha R, Verma V, Kumar A, Chakraborty C, Singh B, Marotta F, Jain S, Yadav H. Cholesterol-lowering probiotics as potential biotherapeutics for metabolic diseases. Exp Diabetes Res. 2012, 2012:902917

Kaushal D, Kansal VK. Age-related decline in macrophage and lymphocyte functions in mice and its alleviation by treatment with probiotic dahi containing lactobacillus acidophilus and bifidobacterium bifidum. J Dairy Res. 2011, 78:404-411

Rodrigues MA, Silva LA, Salvadori DM, De Camargo JL, Montenegro MR. Aberrant crypt foci and colon cancer: Comparison between a short- and medium-term bioassay for colon carcinogenesis using dimethylhydrazine in wistar rats. Braz J Med Biol Res. 2002, 35:351-355

Femia AP, Dolara P, Caderni G. Mucin-depleted foci (mdf) in the colon of rats treated with azoxymethane (aom) are useful biomarkers for colon carcinogenesis. Carcinogenesis. 2004, 25:277-281

Wargovich MJ, Jimenez A, McKee K, Steele VE, Velasco M, Woods J, Price R, Gray K, Kelloff GJ. Efficacy of potential chemopreventive agents on rat colon aberrant crypt formation and progression. Carcinogenesis. 2000, 21:1149-1155

Habig WH, Pabst MJ, Jakoby WB. Glutathione s-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974, 249:7130-7139

de Moreno de LeBlanc A, Perdigon G. Reduction of beta-glucuronidase and nitroreductase activity by yoghurt in a murine colon cancer model. Biocell. 2005, 29:15-24

Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978, 86:271-278

Rasic JL, Kurmann JA. Bifidobacteria and their role. Microbiological, nutritional-physiological, medical and technological aspects and bibliography. Experientia Suppl. 1983, 39:1-295

Kumar A, Singh NK, Sinha PR, Kumar R. Intervention of acidophilus-casei dahi and wheat bran against molecular alteration in colon carcinogenesis. Mol Biol Rep. 2009, 37:621-627

Rafter J. The effects of probiotics on colon cancer development. Nutr Res Rev. 2004, 17:277-284

Balansky R, Gyosheva B, Ganchev G, Mircheva Z, Minkova S, Georgiev G. Inhibitory effects of freeze-dried milk fermented by selected lactobacillus bulgaricus strains on carcinogenesis induced by 1,2-dimethylhydrazine in rats and by diethylnitrosamine in hamsters. Cancer Lett. 1999, 147:125-137

Newell LE, Heddle JA. The potent colon carcinogen, 1,2-dimethylhydrazine induces mutations primarily in the colon. Mutat Res. 2004, 564:1-7

Le Leu RK, Hu Y, Brown IL, Woodman RJ, Young GP. Synbiotic intervention of bifidobacterium lactis and resistant starch protects against colorectal cancer development in rats. Carcinogenesis. 2009, 31:246-251

Kawanishi S, Yamamoto K. Mechanism of site-specific DNA damage induced by methylhydrazines in the presence of copper(ii) or manganese(iii). Biochemistry. 1991, 30:3069-3075

Jackson PE, O'Connor PJ, Cooper DP, Margison GP, Povey AC. Associations between tissue-specific DNA alkylation, DNA repair and cell proliferation in the colon and colon tumour yield in mice treated with 1,2-dimethylhydrazine. Carcinogenesis. 2003, 24:527-533

Pochart P, Marteau P, Bouhnik Y, Goderel I, Bourlioux P, Rambaud JC. Survival of bifidobacteria ingested via fermented milk during their passage through the human small intestine: An in vivo study using intestinal perfusion. Am J Clin Nutr. 1992, 55:78-80

Kanwar SS, Vaiphei K, Nehru B, Sanyal SN. Antioxidative effects of nonsteroidal anti-inflammatory drugs during the initiation stages of experimental colon carcinogenesis in rats. J Environ Pathol Toxicol Oncol. 2008, 27:89-100

Szarka CE, Pfeiffer GR, Hum ST, Everley LC, Balshem AM, Moore DF, Litwin S, Goosenberg EB, Frucht H, Engstrom PF, et al. Glutathione s-transferase activity and glutathione s-transferase mu expression in subjects with risk for colorectal cancer. Cancer Res. 1995, 55:2789-2793

Balasenthil S, Arivazhagan S, Nagini S. Garlic enhances circulatory antioxidants during 7, 12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. J Ethnopharmacol. 2000, 72:429-433

Marteau P, Pochart P, Flourie B, Pellier P, Santos L, Desjeux JF, Rambaud JC. Effect of chronic ingestion of a fermented dairy product containing lactobacillus acidophilus and bifidobacterium bifidum on metabolic activities of the colonic flora in humans. Am J Clin Nutr. 1990, 52:685-688

Rajpal S, Kansal V. Buffalo milk probiotic dahi containing lactobacillus acidophilus, bifidobacterium bifidum and lactococcus lactis reduces gastrointestinal culture induced by dimethylhydrazine dihydrochloride in rats. Milchwissenschaft 2008, 63:122-125

Ling WH, Korpela R, Mykkanen H, Salminen S, Hanninen O. Lactobacillus strain gg supplementation decreases colonic hydrolytic and reductive enzyme activities in healthy female adults. J Nutr. 1994, 124:18-23

Goldin BR, Gorbach SL. Effect of lactobacillus acidophilus dietary supplements on 1,2-dimethylhydrazine dihydrochloride-induced intestinal cancer in rats. J Natl Cancer Inst. 1980, 64:263-265

Hendrickse CW, Kelly RW, Radley S, Donovan IA, Keighley MR, Neoptolemos JP. Lipid peroxidation and prostaglandins in colorectal cancer. Br J Surg. 1994, 81:1219-1223

Skrzydewska E, Stankiewicz A, Michalak K, Sulkowska M, Zalewski B, Piotrowski Z. Antioxidant status and proteolytic-antiproteolytic balance in colorectal cancer. Folia Histochem Cytobiol. 2001, 39 Suppl 2:98-99

Yadav H, Jain S, Sinha PR. Oral administration of dahi containing probiotic lactobacillus acidophilus and lactobacillus casei delayed the progression of streptozotocin-induced diabetes in rats. J Dairy Res. 2008, 75:189-195


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