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Anticarcinogenic Effect of Probiotic Dahi and Piroxicam on DMH-induced Colorectal Carcinogenesis in Wistar Rats

Dheeraj Mohania, Vinod Kumar Kansal, Renu Sagwal, Dilip Shah


This study examined the therapeutic effects of probiotic Dahi containing Lactobacillus acidophilus LaVK2 and Lactobacillus plantarum Lp9, alone or as well as in combination with piroxicam (PXC) as an antioxidant agent in male Wistar rats administered 1, 2-dimethylhydrazine dihydrochloride (DMH). Colorectal carcinogenesis was induced by injecting DMH subcutaneously (40 mg/kg body weight) twice a week for 2 weeks. The rats were fed with buffalo milk or probiotic Dahi alone or in combination with PXC in addition to basal diet ad libitum and euthanized at 8th, 16th and 32nd week of the experiment and examined for biochemical and histopathological profile. A significant reduction in lipid peroxidation measured as thiobarbituric acid reactive substances (TBARS) in liver and colorectal tissue, and carcinogen activating enzyme measured as b-glucuronidase activity in faeces were observed in probiotic Dahi-DMH-PXC group compared with the DMH control group. Probiotic Dahi alone or in combination with PXC was showed a significant (P < 0.05) protective effect by lowering the levels of TBARS, faecal b-glucuronidase and by enhancing the activity GST in liver and colorectal tissues, correlated with decreased tumor incidence, tumor multiplicity and tumor volume in gastrointestinal (GI) tract in DMH induced rats. These observations suggest that probiotic Dahi alone or conjointly with PXC may have therapeutic potential in attenuation of carcinogenesis in GI tract.


Probiotics; Piroxicam; Lactobacillus; Colorectal carcinogenesis

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