Cover Image

Evaluation of invitro Antimutagenic Potential of Lagenaria Siceraria Using Ame’s Test

Jalarambhai Hasmukhlal Thakkar, Patel Chirag A, Santani Devdas D, Jani Girish K

Abstract


Cancer is one of the most life-threatening diseases and widespread in both developed and developing countries. Accumulation of genetic alterations is main etiology for cancer developments. Many of the Cucurbitaceae plants possess antitumor activity traditionally. Methanolic extract of Lagenaria siceraria Standley Fruit was tested for their antimutagenic potential. The extract of plant exhibited varying level of antimutagenicity. Ames test was used in the current study to evaluate antimutagenic activity in TA98 and TA100 strains of Salmonella typhimurium using direct (Sodium azide) acting mutagens. Results of the study showed significant antimutagenicity against mutagen in TA98 and TA100 strains. The antimutagenicity of the extract observed in the present study implies chemopreventive pharmacological importance of Lagenaria siceraria Standley Fruit and encourages its use as a functional food.


Keywords


Antimutagenicity; Sodium azide; Salmonella typhimurium; Ames test; Lagenaria siceraria

References


Doll R, Peto R. The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst. 1981, 66:1191-1308

Raymond JM, Niesink JV, Mannfred AH. Genetic toxicology. In: ph. Van parys., Th M.C.M dekuk, editor. Toxicology principles and applications. 1st ed. CRC press; 1996.p.314-319

Weisburger JH. Antimutagenesis and anticarcinogenesis from the past to future. Mutat Res. 2001, 480-481:23-35

Flora SD, Izzotti A, Agostini FD, Balansky RM, Noonan D, Albini A. Multiple points of intervention in the prevention of cancer and other mutation-related diseases. Mutat Res. 2001, 480:9-22

Flora SD. Mechanisms of inhibitors of mutagenesis and carcinogenesis. Mutat Res. 1998, 402:151-158

Mateuca R, Lombaert N, Aka PV, Decordier I, Kirsch-Volders M. Chromosomal changes: Induction, detection methods and applicability in human biomonitoring. Biochimie. 2006, 88:1515-1531

Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res. 1995, 22:375-383

Kim NY, Song EJ, Kwon DY, Kim HP, Heo MY. Antioxidant and antigenotoxic activities of korean fermented soybean. Food Chem Toxicol. 2008, 46:1184-1189

Santosh KV, Viswanatha GL, Ramesh C, Nandakumar K, Srinath R. Antimutagenic (anticlastogenic) and antioxidant activities of bark extract of Terminalia arjuna. Journal of Genetic Toxicology. 2008, 1:1-7

Harika M, Gasti VD, Shantappa T, Mulge R, Shirol AM, Mastiholi AB et al. Evaluation of bottle gourd genotypes [Lagenaria siceraria (Mol.) Standl.] for various horticultural characters. Karnataka J Agric Sci. 2012, 25:241-244

Mortelmans K, Zeiger E. The ames salmonella/microsome mutagenicity assay. Mutat Res. 2000, 455:29-60

Zahin M, Ahmad I, Aqil F. Antioxidant and antimutagenic activity of carum copticum fruit extracts. Toxicol In Vitro. 2010, 24:1243-1249.

Singh MK, Faisal M, Ahmad A, Sonkar A, Yadav J. Protective effect of lagenaria siceraria against doxorubicin induced cardiotoxicity in wistar rats. International Journal of Drug Development & Research. 2012, 4:298-305

Amit K, Sangh P, Sharma N, Jha KK. Phytochemical, Ethnobotanical and Pharmacological Profile of Lagenaria siceraria : A Review. Journal of Pharmacognosy and Phytochemistry. 2012, 1:27-35


Full Text: PDF

Refbacks

  • There are currently no refbacks.


AJCB (ISSN 2572-5807)Copyright © 2012-2019. All rights reserved. Published by Ivy Union Publishing, 3204 Valley Rush Dr, Apex, North Carolina 27502, United States